Selective toxicity of diphtheria toxin for malignant cells.

Design and Production of Recombinant TAT Protein Structure, Catalytic Domain of Diphtheria Toxin, and Evaluation of Its Effect on Cell Line

Background and Objectives: Cancer is one of the most deadly diseases in the present age and its conventional therapies have had low success. Toxin therapy of cancer is a new therapeutic approach, which has attracted the attention of pharmaceutical specialists. Diphtheria toxin consists of three functional, transducing, and binding domains, that the functional part inhibits protein synthesis and...

Preparation of a low-chloride Medium, for the production of Diphtheria toxin

Sensitivity to diphtheria toxin as a species-specific marker in hybrid cells.

INTRODUCTION It is known that cells from different animal species show marked differences in their susceptibility to the destructive action of diphtheria toxin (Lennox & Kaplan, 1957). It therefore seemed possible that diphtheria toxin might be used as a speciesspecific marker in the study of interspecific hybrid cells and perhaps also as a selective agent to facilitate the isolation of such hy...

Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein.

The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding alpha-melanocyte-stimulating hormone (alpha-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related alpha-MSH hybrid protein in which the dip...

Diphtheria toxin-induced channels in Vero cells selective for monovalent cations.

Ion fluxes associated with translocation of diphtheria toxin across the surface membrane of Vero cells were studied. When cells with surface-bound toxin were exposed to low pH to induce toxin entry, the cells became permeable to Na+, K+, H+, choline+, and glucosamine+. There was no increased permeability to Cl-, SO4(-2), glucose, or sucrose, whereas the uptake of 45Ca2+ was slightly increased. ...