Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.

Alpha-1,3-galactosyltransferase-deficient miniature pigs produced by serial cloning using neonatal skin fibroblasts with loss of heterozygosity

OBJECTIVE Production of alpha-1,3-galactosyltransferase (αGT)-deficient pigs is essential to overcome xenograft rejection in pig-to-human xenotransplantation. However, the production of such pigs requires a great deal of cost, time, and labor. Heterozygous αGT knockout pigs should be bred at least for two generations to ultimately obtain homozygote progenies. The present study was conducted to ...

Cloning of Homozygous 1,3-Galactosyltransferase Gene Knock-Out Pigs by Somatic Cell Nuclear Transfer

Creating genetically modified pigs by using nuclear transfer

Nuclear transfer (NT) is a procedure by which genetically identical individuals can be created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation and treatment of donor cells, artificial activation of reconstructed oocytes, embryo culture and embryo transfer, has been intensively studied in recent years, resulting in birth of cloned pigs in many labs. While i...

Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning

Gene-knockout pigs hold great promise as a solution to the shortage of organs from donor animals for xenotransplantation. Several groups have generated gene-knockout pigs via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and somatic cell nuclear transfer (SCNT). Herein, we adopted a simple and micromanipulator-free method, handmade cloning (HMC) i...

The use of fish in xenotransplantation research.

quires intelligent use of comparative biological knowledge. Even though the pig clearly stands out as the animal, which is most suited as donor of organs and tissues within human medicine, primitive vertebrates, such as fish, may contribute to research relevant to the area of xenotranplantation, and even in some cases serve as tissue donors. As a part of molecular humanization of swine tissue, ...