Effect of Purine Nucleoside Analogue-Acyclovir on The Sperm Parameters and Testosterone Production in Rats
نویسندگان
چکیده
BACKGROUND Acyclovir (ACV), a synthetic purine nucleoside analogue derived from guanosine, is known to be toxic to gonads and the aim of this study was to evaluate the effect of ACV on the sperm parameters and testosterone production in rat. MATERIALS AND METHODS In this experimental study, forty adult male Wistar rats (220 ± 20 g) were randomly divided into five groups (n=8 for each group). One group served as control and one group served as sham control [distilled water was intraperitoneally (i.p.) injected]. ACV was administered intraperitoneally in the drug treatment groups (4, 16 and 48 mg/kg/day) for 15 days. Eighteen days after the last injection, rats were sacrificed by CO2 inhalation. After that, cauda epididymides were removed surgically. At the end, sperm concentrations in the cauda epididymis, sperm motility, morphology, viability, chromatin quality and DNA integrity were analyzed. Serum testosterone concentrations were determined. RESULTS The results showed that ACV did not affect sperm count, but decreased sperm motility and sperm viability at 16 and 48 mg/kg dose-levels. Sperm abnormalities increased at 48 mg/kg dose-level of ACV. Further, ACV significantly increases DNA damage at 16 and 48 mg/kg dose-levels and chromatin abnormality at all doses. Besides, a significant decrease in serum testosterone concentrations was observed at 16 and 48 mg/ kg doses. CONCLUSION The present results highly support the idea that ACV induces testicular toxicity by adverse effects on the sperm parameters and serum level of testosterone in male rats.
منابع مشابه
Effect of Purine Nucleoside Analogue-Acyclovir on The Sperm Parameters and Testosterone Production in Rats
متن کامل
P-52: Acyclovir Induced Sperm and Hormonal Disorders in Male Rats
Background: Acyclovir is asynthetic purin nucleoside analog,which has been reported to be very effective against the treatment of herpes simplex and varicella zoster infections. Acyclovir is known to be toxic to gonads, but available literature fails to provide the indepth analyses of damage.The aim of the present study was to evaluate the effect of Acyclovir on DNA damage and nuclear maturatio...
متن کاملP-51: A Purine Nucleoside Analogue_Acyclovir Impairs Testicular Functions in Male Rats
Background: Acyclovir (ACV) is a synthetic purine nucleoside analog drived from guanosine. ACV possesses antiviral activity against Herpes Simplex Virus types 1 and 2 (HSV-1 and HSV-2 ), Varicella Zoster virus (VZV) , Epstein Barr virus (EBV) and Cytomegalovirus (CMV). ACV exert its effects by interfering with DNA synthesis,thus inhibiting viral replication. ACV is khown to be toxic to gonads,t...
متن کاملToxic effect of acyclovir on testicular tissue in rats
BACKGROUND Acyclovir (ACV), a synthetic purine nucleoside analogue, is known to be toxic to gonads. OBJECTIVE The current study evaluated cytotoxicity of ACV on histopathological changes in testis tissue and serum testosterone and lipid peroxidation concentrations of male rats. MATERIALS AND METHODS Animals were divided into five groups. One group served as control and one group served as c...
متن کاملP-230: Low Pregnancy Rate in Female Rats Mated by Male Rats Exposed to Acyclovir
Background: Acyclovir [9-(2-hydroxyethoxymethyl)guanine; ACV], an acyclic nucleoside analogue, has shown a potent antiviral activity. It is known to inhibit the replication of herpes viruses including herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), varicellazoster virus, and Epstein-Barr virus in cell cultures and in animals. ACV is khown to be toxic to gonads,then the aim of the presen...
متن کامل