A slide culture method for streptomycin sensitivity testing.
نویسندگان
چکیده
Ever since Koch first cultivated the tubercle bacillus, methods have been sought to hasten its growth (in vitro). In his original communication Koch’ described a technique of examining cultures of tubercle bacilli grown in coagulated blood serum placed in watch glasses or hollow glass slides. He examined these preparations by low-power magnification and was able to detect growth in one week. Pryce2 in 1941, stimulated interest in a slide-culture method for cultivating tubercle bacilli in sputum. He made slide cells by placing a glass ring over a micro-slide to which a liquid medium was added. With this method colony formation was visible after only three or four days. Rosenberg3 modified this technique by decontaminating the sputum smears with acid and entirely immersing the slides in tubes of liquid medium. Muller4 also described a modification of Pryce’s technique which he used to estimate the bacteriostatic power of chemicals on the tubercle bacillus. Berry and Lowry1 reported early growth of tubercle bacilli from pathological material with a similar technique and gave an excellent review of the slide culture literature.
منابع مشابه
Sensitivity Testing A Slide Culture Method for Streptomycin
1950;17;202-205 Dis Chest SCHWARTZ MARTIN M. CUMMINGS, MARGARET C. DRUMMOND and HARRIET B. Sensitivity Testing A Slide Culture Method for Streptomycin http://chestjournal.chestpubs.org/content/17/2/202 can be found online on the World Wide Web at: The online version of this article, along with updated information and services ) ISSN:0096-0217 http://chestjournal.chestpubs.org/site/misc/reprints...
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ورودعنوان ژورنال:
- Diseases of the chest
دوره 17 2 شماره
صفحات -
تاریخ انتشار 1950