Demonstration of delta rec-pseudo J alpha rearrangement with deletion of the delta locus in a human stem-cell leukemia

نویسندگان

  • C G Begley
  • P D Aplan
  • M P Davey
  • J P de Villartay
  • D I Cohen
  • T A Waldmann
  • I R Kirsch
چکیده

It has been hypothesized that a rearrangement between the delta recombining element (delta Rec) and a pseudo J alpha gene serves to delete the TCR-delta locus before rearrangement of the TCR-alpha genes. We have now sequenced a direct, site-specific rearrangement between the delta Rec element and a pseudo J alpha gene in a human leukemic stem-cell line. Putative "N-sequence" addition was noted at the site of recombination, suggesting that this event occurred at a time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-delta locus is required before rearrangement of the TCR-alpha chain genes.

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 170  شماره 

صفحات  -

تاریخ انتشار 1989