Simple liquid-chromatographic measurement of vanillylmandelic acid and homovanillic acid in urine on filter paper for mass screening of neuroblastoma in infants.
نویسندگان
چکیده
We describe a simple and rapid method for measuring catecholamine metabolites in urine collected on filter paper. By this method, "high-performance" liquid chromatography, urinary vanillylmandelic acid and homovanillic acid can be measured within 15 min after being eluted from filter paper treated with tartrate buffer. One hundred urine samples can be pretreated within 2 h and 100-120 samples analyzed at 18-min intervals overnight in two chromatographic systems. Moreover, urinary creatinine also can be measured rapidly because of the use of microtiter plates. We applied this method in a mass screening program for early detection of neuroblastoma in infants. Between April 1986 and March 1987, two patients with neuroblastoma were detected. In all, neuroblastoma has been detected in 17 of 88,887 infants so examined since April 1981.
منابع مشابه
DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY
We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...
متن کاملScreening for neuroblastoma in the north of England.
OBJECTIVE To determine the feasibility of establishing a system of screening for neuroblastoma. DESIGN Prospective study of mass screening in four clearly defined geographical areas. SETTING Four health districts of the Northern region of England. SUBJECTS 20,829 babies aged 6 months, 92% of target population. INTERVENTIONS Collection of urine on filter paper for analysis of content of ...
متن کاملDirect measurement of homovanillic, vanillylmandelic and 5-hydroxyindoleacetic acids in urine by capillary electrophoresis.
Separation conditions in CE, with a neutral coated capillary and reversed polarity, have been optimised to make direct measurement of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid possible in urine samples without pre-treatment. The method developed has been validated, presenting adequate parameters for linearity, accuracy and precision. Detection limits range from 0.0...
متن کاملOne SLE to measure them all: Two-part elution for analysis of urinary HIAA, HVA and VMA
BACKGROUND: The monoamine acids 5’-hydroxyindoleacetic (HIAA), homovanillic (HVA), and vanillylmandelic (VMA) are metabolites of serotonin and the catecholamine neurotransmitters epinephrine, norepinephrine, and dopamine. Laboratory measurement of these acids in urine is used to assess overproduction of the parent amines due to carcinoid and neuroendocrine tumors (neuroblastoma, pheochromocytom...
متن کاملSensitive, rapid and easy analysis of three catecholamine metabolites in human urine and serum by liquid chromatography tandem mass spectrometry.
A sensitive and easy analytical method for catecholamine metabolites including 4-hydroxy-3-methoxyphenylglycol sulfate (HMPG sulfate), vanillylmandelic acid (VMA) and homovanillic acid (HVA) determination was developed based on liquid chromatography-tandem mass spectrometry in a negative multiple reaction monitoring mode. The analytes were rapidly separated on a reversed-phase Waters Xbridge C1...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 33 11 شماره
صفحات -
تاریخ انتشار 1987