Immunoperoxidase localization of colonic mucoprotein antigen in neoplastic tissues.

Immunoperoxidase localization of a high-molecular-weight mucin recognized by monoclonal antibody 1D3.

The distribution of an antigen recognized by murine monoclonal antibody 1D3 (Bhattacharya, M., Chatterjee, S.K., Barlow, J. J., and Fuji, H. Cancer Res., 42: 1650-1654, 1982) was investigated in various types of human malignant and normal adult tissues by indirect immunoperoxidase assay in fixed paraffin-embedded sections. One hundred percent of ovarian mucinous cystadenocarcinomas expressed hi...

A new antigen on the epithelial membrane : its immunoperoxidase localization in normal and neoplastic tissue

Using antisera raised against defatted human cream we have demonstrated a new antigen on the epithelial membrane. An indirect immunoperoxidase technique on routinely processed histological sections was used, and we describe results which show that this antigen has a widespread but highly selective distribution, being apparently localised to membranes that have a secretary function. The antigen ...

Gastrointestinal Cancer-associated Antigen in Immunoperoxidase Assay1

A monosialoganglioside antigen of gastrointestinal adenocarcinomas defined by murine monoclonal antibody was dem onstrated by immunoperoxidase (IP) assay in fixed paraffinembedded tumors in 59% of colonie adenocarcinomas, 86% of pancreatic adenocarcinomas, and 89% of all gastric ade nocarcinomas. In all patients with detectable levels of antigen in circulation, the resected tumors also expresse...

The Use of indirect Immunoperoxidase Assay in Diagnosis of Type A (H9N2) Avian Influenza Virus Antigen on Frozen Tissue Sections

Comparison of human colonic mucoprotein antigen from normal and neoplastic mucosa.

Colonie mucoprotein antigen (CMA), a high-molecularweight, tissue-specific mucin, was obtained from 9 normal mucosa! samples and 12 colonie adenocarcinomas. Puri fication was achieved by means of phenokwater extrac tion followed by ethanol precipitation and molecular sieve chromatography. Chemical analyses of the individual nor mal CMA preparations indicated similar qualitative and quantitative...