Isolation and Quantification of Aflatoxin from Aspergillus Flavus Infected Rice
نویسندگان
چکیده
Isolation of Apergillus flavus from groundnut and production of aflatoxin on rice. a method has been developed for the production of aflatoxin by growing Aspergillus flavus strain AJ on the solid substrate rice. Optimal yields, more than 1mg of aflatoxin B1 per gram of starting material, were obtained in 5 days at 28C.A crude product containing aflatoxin was isolated by HPLC (High Performance Liquid Chromatography). The cruded product consists of 55% aflatoxin in the following ratio:B1-B2-G1G2,100:0.15:0.22:0.02.Aflatoxin B1 was separated from all the impurities and from the other aflatoxins by chromatograph on silica gel with 1% ethyl alcohol in chloroform.
منابع مشابه
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