Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F
نویسنده
چکیده
Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.
منابع مشابه
Ethanol Extract of Tripterygium wilfordii Hook. f. Induces G0/G1 Phase Arrest and Apoptosis in Human Leukemia HL-60 Cells Through c-Myc and Mitochondria-Dependent Caspase Signaling Pathways
Tripterygium wilfordii Hook. f. is a traditional Chinese herb (Murphy, 2006; Qiu et al., 2003). The extract of Tripterygium wilfordii Hook. f. has been widely applied to the treatment of immune-related diseases, such as rheumatoid arthritis (RA), nephritis, and systemic lupus erythematosus (SLE) (Chang et al., 1999; Wang et al., 2000). Extracts of Tripterygium wilfordii Hook. f. have been shown...
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Objective: Triptolide, the active component of Tripterygium wilfordii Hook F has been used to treat autoimmune and inflammatory conditions for over two hundred years in traditional Chinese medicine. However, the processes through which triptolide exerts immunosuppression and anti-inflammation are not understood well. In this review, we discuss the autoimmune disorders and inflammatory condition...
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