CT 2.4.4 Discovering Mammalian Endocytic Pathways with High-Throughput Image- Based siRNA Screens and Network Analysis
نویسندگان
چکیده
Introduction Research on membrane trafficking has generated many key insights into the molecular machinery of individual events. However, comprehensive and quantitative models that describe membrane trafficking in a mammalian cell as one system are lacking. The main workhorses to discover these pathways in this research are mammalian viruses, which have co-evolved with mammalian cells and, therefore, know all the entry routes and organelles constituting the endocytic membrane system. Viruses are easily visualized and followed by means of various light microscopy techniques. In addition, successful infectious entry is an easy and robust readout for high-throughput screens. (Pelkmans et al (2005))
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