OSSC1E-K19, a novel phytochemical component of Osteomeles schwerinae, prevents glycated albumin-induced retinal vascular injury in rats
نویسندگان
چکیده
In the pathophysiology of diabetic retinopathy (DR), advanced glycation end products (AGEs) and vascular endothelial growth factor (VEGF) are thought to have important roles. It is known that VEGF causes a breakdown of the blood‑retinal barrier (BRB) and retinal neovascularization; however, how AGEs affect the retina has largely remained elusive. OSSC1E‑K19 is a novel phytochemical component of Osteomeles schwerinae. The objective of the present study was to evaluate the protective effects of OSSC1E‑K19 on retinal vascular injury in AGE‑modified rat serum albumin (AGE-RSA)-induced retinopathy. AGE-RSA-injected rat eyes were used investigate the protective effects of OSSC1E‑K19 on BRB breakdown. Intravitreal injection of OSSC1E-K19 prevented AGE-RSA-induced BRB breakdown and decreased retinal VEGF expression in retinal vessels. In addition, OSSC1E-K19 inhibited the loss of occludin, a significant tight junction protein. These results supported the potential therapeutic utility of OSSC1E-K19 for retinal vascular permeability diseases.
منابع مشابه
Cytotoxic Effect of \" Glycated Albumin-Transition Metal Ion\" on Rat Hepatocyte Suspension
Background: Combination of glycation and oxidation is associated with diabetes mellitus. The aim of this study was to clarify the effect of glycated proteins in presence of transition metal ions on production of reactive oxygen species (ROS) in rat hepatocyte suspension. Methods: Glycated albumin was prepared by incubation of bovine serum albumin with 100 mM glucose in 0.3 M phosphate buffer a...
متن کاملRetinal and renal vascular permeability changes caused by stem cell stimulation in alloxan-induced diabetic rats, measured by extravasation of fluorescein.
AIM To determine whether treatment with the stem cell stimulator Olimpiq® Stem×Cell prevents increase of retinal and renal vascular permeability in alloxan-induced diabetic rats. MATERIALS AND METHODS Two groups of Wistar rats were made diabetic by single intraperitoneal injection of Alloxan. The third, the control group, received vehicle alone. One diabetic group received Olimpiq® Stem×Cell ...
متن کاملThe Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress
Neuropilin-1 (NRP-1) is a transmembrane glycoprotein. As a VEGF co-receptor, NRP1 significantly enhances VEGFR2 signaling and promotes vascular permeability and migration. The purpose of this study was to evaluate the effects of an NRP-1 inhibitor, ATWLPPR peptide, on the early stages of diabetic retinopathy. Eight-week-old male C57BL/6 mice were divided into three groups: a Normal group, a Dia...
متن کاملاثر گلوتامین بر شاخص های استرس اکسیداتیو، التهابی گلیکه و همچنین فعالیت سیستم گلیاوکسیلاز در موش های صحرایی دیابتی- آترواسکلروزی
Background and purpose: Vascular complications of diabetes are the most common causes of mortality in diabetic patients. Hyperglycemia, insulin resistance, dyslipidemia, glycation products, oxidative stress, and inflammation lead to atherosclerosis and diabetic nephropathy in diabetes. This research aimed at studying the effect of glutamine (Gln) on main causes of vascular complications in diab...
متن کاملExtract of Adenanthera pavonina L. seed reduces development of diabetic nephropathy in streptozotocin-induced diabetic rats
Objective: The aim of the present study was to investigate the renal protective effect of Adenanthera pavonina (A. pavonina) seed aqueous extract (APSAE), in streptozotocin (STZ)-induced diabetic rats. Materials and Methods: The renal protective effect of A. pavonina seed aqueous extract (APSAE) was studied in STZ-induced diabetic rats. APSAE (50, 100 and 200 mg/kg per day) was given daily to ...
متن کامل