An enrichment isolation procedure for minor Bacillus populations.

Isolation and preliminary characterization of bacteriophages for Bacillus subtilis.

Romig, W. R. (University of California, Los Angeles), and A. M. Brodetsky. Isolation and preliminary characterization of bacteriophages for Bacillus subtilis. J. Bacteriol. 82:135-141. 1961.-A simplified procedure for direct isolation of phages for Bacillus subtilis from soil was developed. Phage enrichment was accomplished by growing streptomycin-resistant B. subtilis in medium previously inoc...

Use of the beta-lactamase inhibitor clavulanic acid in the isolation of auxotrophic mutants of Bacillus licheniformis.

The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment.

Isolation and Identification of an Alkaline Protease Producing Bacillus from Soil

A New Enzymatic Method for Rapid Diagnosis of Phenylketonuria Using Alkaliphilic Bacillus

Rapid and in time diagnosis of phenylketonuria (PKU) in affected infants can help preventing the progress of mental and developmental disorders associated with the disease. Here we report the isolation of alkaliphilic Bacillus bacteria capable of producing high level of Phenylalanine dehydrogenase (PheDH) from soil. A new quantitative and rapid test for PKU diagnosis was then developed using th...

O-12: Clinical Grade Isolation and Cryopreservation of Human Testicular Cells

Background: More than a decade work in preservation of male fertility by spermatogonial stem cell (SSC) transplantation in animal models as well as new discoveries about the nature of human SSCs has prompted an investigation into the possibility of an effective clinicalgrade procedure for isolation and cryopreservation of human testicular cells. Materials and Methods: Clinical-grade reagents, v...