Counting and Imaging Bacteria on Mineral Surfaces
نویسندگان
چکیده
Direct Counting 45 Procedure 1: DAPI Cell Count: Sandy Sediment 46 Rock MPN 47 SEM Surface Counts 48 Quantitative FISH 48 Procedure 2: Sample Collection and Preservation for FISH 50 Procedure 3: Slide Preparation and Cell Fixation for FISH 50 Procedure 4: Hybridization 51 Procedure 5: Post-hybridization 52 Procedure 6: Examination and Quantification 53 Chemical Biomass 53 Procedure 7: Lipid Phosphate Extraction and Measurement 54
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