A study of fluorescence emission intensity of reaction products between selected amino acids and DFO, 1,2-indanedione and 1,2-indanedione – zinc chloride

chloride or cadmium nitrate. However, the complexation was secondary in relation to ninhydrin-amino acid reaction [4]. At the end of the 1990’s, a new component named 1,2-IND (1,2-indanedione) was applied, which reacted with amino acids and with DFO, whereby the reaction product exhibited fluorescent properties [5, 6]. The follow-up scientific activity within this scope was focused on the optimization of solution components and the use of new solvents [7, 8, 9, 10, 11]. A remarkable achievement of the works towards increased sensitivity of the detection methods was the inclusion of zinc chloride (ZnCl2) into the working solution of 1,2-IND [12]. This article presents the results of the studies on fluorescence intensity of the products of the reaction between nine selected amino acids and DFO, 1,2-indanedione and 1,2-indanedione – zinc chloride, deposited onto absorptive surface in the form of printing paper and filter paper. At the same time, the present work is a follow-up on previous studies on fluorescence spectral characteristics of amino acids reacting with DFO, 1,2-indanedione and 1,2-indanedione – zinc chloride deposited onto absorptive surface, over time [13]. Introduction