Propagation and Bulb Formation of Fritillaria (fritillaria Imperialis L.) via in Vitro Culture

The Fritillariaimperialis is grown natively in Iran. Between ornamental bulblet plants, this plant has special place, therefore this plant needs more attention, otherwise this plant will be destroyed in future. For this reason in vitro culture of Fritillariaimperialis was studied. Tissue culture techniques have high potential for mass propagation. The multiplication efficacy of the method by bulb is low; the plantlets are easy to get diseases and depended on the crop. Plant tissue culture is a technique that has ability of production of a large number of high quality plants. Factors influencing on the regeneration ability and in vitro plantlet growth were examined. The number of regenerated shoots was the highest on MS medium supplemented with 0.5 mg/l TDZ and 30 g/l sucrose; the number of roots was the highest on MS medium supplemented with 0.2 mg/l NAA and 30 g/l sucrose; the in vitro bulb diameter is largest. In vitro plantlets survived in greenhouse at a high rate. A whole process was established for rapid multiplication of Fritillariaimperialis L.