Cost-benefit analysis of a method using diatomaceous earth to purify Tamm-Horsfall protein.
نویسندگان
چکیده
منابع مشابه
Evaluation of Glycan Profiles of Tamm-horsfall Glycoprotein and Uromodulin
Uromodulin is well-known to be the more potent immunosuppressive glycoprotein isolated from pregnant women urine, compared to its counterpart, Tamm-Horsfall glycoprotein (THP) isolated from non-pregnant women/male urine. However, structural profiles of these glycoproteins were controversial until today. Hence, this paper aims to identify the pregnancy associated glycosylation changes of THP and...
متن کاملImmunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin.
BACKGROUND Tamm-Horsfall glycoprotein (THP) and uromodulin are the most abundant glycoproteins in non-pregnant women's/men's and pregnant women's urine, respectively. However, the bioactivities of these glycoproteins are still unclear. OBJECTIVE To evaluate the immunomodulatory properties of THP and uromodulin on human peripheral blood mononuclear cells (PBMC) METHODS: THP and uromodulin isol...
متن کاملIncreased urinary levels of Tamm-Horsfall glycoprotein suggest a systemic etiology of interstitial cystitis.
PURPOSE We investigated the role of Tamm-Horsfall protein in interstitial cystitis. MATERIALS AND METHODS Urinary Tamm-Horsfall protein excretion was analyzed in interstitial cystitis patients and controls, and bladder biopsy specimens were stained immunohistochemically for Tamm-Horsfall protein. RESULTS Urinary Tamm-Horsfall protein levels in 28 women with interstitial cystitis were statis...
متن کاملRole of Tamm-Horsfall protein and uromodulin in calcium oxalate crystallization.
One of the defenses against nephrolithiasis is provided by macromolecules that modulate the nucleation, growth, aggregation and retention of crystals in the kidneys. The aim of the present study was to determine the behavior of two of these proteins, Tamm-Horsfall and uromodulin, in calcium oxalate crystallization in vitro. We studied a group of 10 male stone formers who had formed at least one...
متن کاملEffect of Tamm-Horsfall protein on calcium oxalate precipitation.
The effect of Tamm-Horsfall protein isolated from urine of healthy subjects on calcium oxalate precipitation was studied in model systems of precipitation. The study was performed using following conditions: concentrations of calcium chloride 10 mmol/l, sodium chloride 150 mmol/l, oxalic acid 300 mumol/l; pH 6.0, and temperature 310 K. The concentration of Tamm-Horsfall protein varied between 1...
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ورودعنوان ژورنال:
- BioTechniques
دوره 36 1 شماره
صفحات -
تاریخ انتشار 2004