A spectrophotometric assay for feruloyl esterases.

نویسندگان

  • Vladimír Mastihuba
  • Lubomír Kremnický
  • Mária Mastihubová
  • J L Willett
  • Gregory L Côté
چکیده

We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.

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عنوان ژورنال:
  • Analytical biochemistry

دوره 309 1  شماره 

صفحات  -

تاریخ انتشار 2002