Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression.

نویسندگان

  • Noelia Fradejas
  • Bradley A Carlson
  • Eddy Rijntjes
  • Niels-Peter Becker
  • Ryuta Tobe
  • Ulrich Schweizer
چکیده

Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA([Ser]Sec), including N(6)-isopentenylation of base A(37). We show that Trit1 is a dimethylallyl:tRNA([Ser]Sec) transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [(14)C]dimethylallyl pyrophosphate to tRNA([Ser]Sec). 37A>G tRNA([Ser]Sec) is resistant to isopentenylation by Trit1.

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عنوان ژورنال:
  • The Biochemical journal

دوره 450 2  شماره 

صفحات  -

تاریخ انتشار 2013