The Need for a PARP in vivo Pharmacodynamic Assay - Update 2010
نویسنده
چکیده
Trevigen PARP Pharmacodynamic Assay II. In this article we present information regarding improvements to Trevigen’s PARP Pharmacodynamic Assay discussed last year. We provide validation data and accelerated shelf life data regarding key assay components for the new assay format which features: a 96 well assay plate pre-coated with PAR capture antibody, extended dynamic range, and high signal to noise ratios. For readers that missed the discussion of the first assay we have retained much of the same basic information describing the role of PARP and DNA repair.
منابع مشابه
The Need for a PARP in vivo Pharmacodynamic Assay
Poly(ADP-ribose) polymerases are promising therapeutic targets. In response to DNA damage, poly(ADP-ribose) polymerases-1and 2 (PARP-1, PARP-2) are rapidly activated by DNA strand breaks. Once activated, NAD + is consumed for the synthesis of highly negatively charged polymers of ADPribose (PAR) on target nuclear proteins that include PARP-1 itself as a major acceptor [1, 2]. These highly branc...
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تاریخ انتشار 2010