Nucleopolyhedrovirus: scanning electron microscopy technique.
نویسندگان
چکیده
A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis revealed a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identified it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specific for each viral lineage.
منابع مشابه
Morphological Characterization of Combustion Deposited Diamond Crystals and Films
Single crystals and polycrystalline diamond films of several thicknesses were deposited using oxygen/acetylene combustion flame technique. The substrate used was pure polycrystalline molybdenum subjected to mechanical polishing. Quality and microstructural characteristic of diamond produced were investigated using X-Ray diffraction, Raman Spectroscopy, Scanning and Transmission Electron Microsc...
متن کاملNano-structural Characterization of Post-annealed ZnO Thin Films by X-ray Diffraction and Field Emission Scanning Electron Microscopy
ZnO thin films were deposited on Si(400) substrates by e-beam evaporation technique, and then post-annealed at different annealing temperatures (200-800°C). Dependence of the crystallographic structure, nano-strain, chemical composition and surface physical Morphology of these layers on annealing temperature were studied. The crystallographic structure of films was studied using X-Ray Diffracti...
متن کاملBibliography of environmental scanning electron microscopy.
Two updated lists of publications on environmental scanning electron microscopy are compiled. One list contains mainly those papers dealing with the development and instrumentation, while the other deals mainly with the applications of the technique. A brief introductory summary of the field is presented.
متن کاملStudy of Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM)
Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) are widely used in material science, metallurgy sicence and life science researches. TEM is an imaging technique where a beam of electrons is focused onto a specimen causing an enlarged version to appear on a fluorescent screen or layer of photographic film. SEM is a technique of electron microscope to produce high re...
متن کاملMethods for Scanning Transmission Electron Microscopy High Angle Annular Dark Field Based for Three Dimensional Analysis of the Local Composition in Solid Alloys
This paper reports on a novel approach to quantitatively reconstruct the number and the three-dimensional distribution of guest atoms inside a host matrix by a Scanning Transmission Electron Microscopy High Angle Annular Dark Field technique. Since the position of the guest atoms in the column strongly affects the chemical quantification for each column [1][2] this technique allows in addition ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Neotropical entomology
دوره 35 6 شماره
صفحات -
تاریخ انتشار 2006