Isolation and characterization of novel microsatellite markers from Siamese fighting fish (Betta splendens, Osphronemidae, Anabantoidei) and their transferability to related species, B. smaragdina and B. imbellis.

نویسندگان

  • V Chailertrit
  • A Swatdipong
  • S Peyachoknagul
  • J Salaenoi
  • K Srikulnath
چکیده

Ten novel microsatellite markers were developed and characterized from Siamese fighting fish (Betta splendens). Nine of ten markers were polymorphic, exhibiting an allelic number (NA) from 2 to 6 alleles per locus. The effective number of alleles (NE) ranged from 1.60 to 3.08 (average of 2.30). The observed (HO) and expected (HE) heterozygosities ranged from 0.13 to 0.67 (average of 0.39) and 0.29 to 0.63 (average of 0.50), respectively. Linkage disequilibrium was not significantly detected for any pair of loci, and only two loci (BettaMS23 and BettaMS28) showed significant deviations from Hardy-Weinberg expectations. Of these, six loci could be amplified in genomic DNA of the closely related species B. imbellis and three loci in B. smaragdina. These microsatellite markers could be used as a tool to investigate genetic diversity and population structure, as well as breeding programs in hatcheries.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Virulence of Aeromonas hydrophila in Siamese fighting fish (Betta splendens) and the bacterium susceptibility to some herbal plants

Aeromonas hydrophila was isolated from diseased Siamese fighting fish Betta splendens Regans.  The virulence of A. hydrophiala to Siamese fighting fish was conducted by intraperitoneal injection of two doses of bacterial suspensions with 7.5x107 and 7.5x105 cfu per 0.05 ml. The cumulative mortality was observed in high and low dose groups which showed 98.33 and 20 percent, respectively. Moreove...

متن کامل

Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences☆

In Thailand, there are currently five recognized species members of the bubble-nesting Betta genus, namely Betta splendens, B. smaragdina, B. imbellis, B. mahachaiensis and B. siamorientalis. In 2010, we indicated the possibility, based on COI barcoding evidence, that there might be two additional species, albeit cryptic, related to the type-locality B. smaragdina in some provinces in the north...

متن کامل

Virulence of Aeromonas hydrophila in Siamese fighting fish (Betta splendens) and the bacterium susceptibility to some herbal plants

Aeromonas hydrophila was isolated from diseased Siamese fighting fish Betta splendens Regans.  The virulence of A. hydrophiala to Siamese fighting fish was conducted by intraperitoneal injection of two doses of bacterial suspensions with 7.5×107 and 7.5×105 cfu per 0.05 ml. The cumulative mortality was observed in high and low dose groups which showed 98.33 and 20 percent, respectively. Moreove...

متن کامل

Male 11-ketotestosterone levels change as a result of being watched in Siamese fighting fish, Betta splendens.

This study investigated the effects of nesting status and the presence of an audience on 11-ketotestosterone (11KT) levels in male Siamese fighting fish, Betta splendens. Prior studies have demonstrated that both nesting status, an indicator of territory-holding power and reproductive state, and the sex of a conspecific audience lead to differences in male behavior during aggressive encounters....

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Genetics and molecular research : GMR

دوره 13 3  شماره 

صفحات  -

تاریخ انتشار 2014