Effects of thiopental anesthesia on local rates of cerebral protein synthesis in rats

Smith, C. Beebe, C. Eintrei, J. Kang, and Y. Sun. Effects of thiopental anesthesia on local rates of cerebral protein synthesis in rats. Am. J. Physiol. 274 (Endocrinol. Metab. 37): E852–E859, 1998.—We have examined the effects of a surgical level of thiopental anesthesia in adult male rats on local rates of cerebral protein synthesis with the quantitative autoradiographic L-[1-14C]leucine method. The relative contribution of leucine derived from protein breakdown to the intracellular precursor amino acid pool for protein synthesis was found to be statistically significantly decreased in the anesthetized rats compared with controls. In the brain as a whole and in 30 of the 35 brain regions examined, rates of protein synthesis were decreased (1–11%) in the anesthetized rats. Decreases were statistically significant (P # 0.05) in the brain as a whole and in six of the regions, and they approached statistical significance in an additional 13 regions, indicating a tendency for a generalized but small effect.