Antioxidant enzymatic defences in human follicular fluid: characterization and age-dependent changes.

نویسندگان

  • M C Carbone
  • C Tatone
  • S Delle Monache
  • R Marci
  • D Caserta
  • R Colonna
  • F Amicarelli
چکیده

The aim of this work was to study the antioxidant enzymatic defences in human follicular fluid and investigate their possible changes during reproductive ageing. To this end, we tested the specific activities and protein expression of enzymes involved in reactive oxygen species (ROS) scavenging and in detoxification of ROS byproducts in follicular fluid from young (range 27-32 years, n = 12) and older (range 39-45 years, n = 12) women participating in an IVF programme. Results show that all the tested enzymes [superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione transferase, glutathione reductase] were significantly expressed in human follicular fluid. However, when the two age groups were compared, we found that follicular fluid from older women exhibited a reduced level of glutathione transferase and catalase activities and a higher level of SOD activity. Immunoblot analysis revealed that ageing was associated with decreased protein expression of GST Pi isoform and did not affect SOD and catalase protein expression. Taken together, these findings indicate that reproductive ageing is accompanied by a change in the antioxidant enzymatic pattern that could impair ROS scavenging efficiency in the follicular environment.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Age-dependent allocation of carotenoids to coloration versus antioxidant defences.

Aging is commonly attributed to age-related changes in oxidative damage due to an increased production of reactive oxygen species (ROS) and a weakened efficacy of enzymatic antioxidants. These age-related changes might therefore modify the use of dietary antioxidants, including carotenoids. As carotenoids are closely associated with the expression of secondary sexual signals, the allocation of ...

متن کامل

O-26: Association of Follicular Fluid Endocrine Changes with Oocyte Morphology and Quality in PCOS Patients Undergoing Metformin and N-Acetylcysteine Treatment

Background: Polycystic ovary syndrome (PCOS) is a common cause of infertility often responsive to treatment with oral ovulation induction agents. PCOS patients are typically characterized by producing an increased number of oocytes that they are often of poor quality, leading to lower fertilization, cleavage and implantation rates. Recently data involve functional associations between endocrine...

متن کامل

Fatty Acid Composition of Human Follicular Fluid Phospholipids and Fertilization Rate in Assisted Reproductive Techniques

Background: Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...

متن کامل

P-59: The Association between Levels of Vitamin E in Follicular Fluid with The Morphology of Oocytes and The Quality of Embryos in Patients with IVF

Background According to the global increasing trend of infertility, using assisted reproductive technologies to solve this issue is recommended. It seems that vitamin E is an antioxidant with protective role in the body. Therefore, we aimed to investigate the relation between levels of vitamin E in Follicular fluid with the morphology of oocytes and the quality of embryos in patients with IVF. ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Molecular human reproduction

دوره 9 11  شماره 

صفحات  -

تاریخ انتشار 2003