Proteins – structure, properties and their separation by SDS- polyacrylamide gel electrophoresis
نویسندگان
چکیده
This paper deals with three basic aspects: 1) basics of protein structure in relation to physicochemical characterization, protein stability, importance of disulfide bond and their structure; 2) basic principles involved in polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE and the rationale behind the use of disc electrophoresis; and 3) detailed protocol involved in SDS-PAGE in terms of reagent preparation, sample preparation and loading, staining and destaining, calculation of relative mobility and pairwise comparisons between species.
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