Finding gene-expression patterns in bacterial biofilms.

Evaluation of the Expression of NLRP1 Inflammasome in Patients with Bacterial Septicemia

Background: Septicemia is the most important cause of mortality, especially in hospitalized patients, due to the influence of the immune response by infection. NLRP1 (Nod-like receptor P1) is an intracellular receptor that recognizes microbial-dependent molecular patterns. The main intracellular mechanism of anti-septicemia is still being investigated. The purpose of this study was to evaluate ...

The amino acid valine is secreted in continuous-flow bacterial biofilms.

Biofilms are structured communities characterized by distinctive gene expression patterns and profound physiological changes compared to those of planktonic cultures. Here, we show that many gram-negative bacterial biofilms secrete high levels of a small-molecular-weight compound, which inhibits the growth of only Escherichia coli K-12 and a rare few other natural isolates. We demonstrate both ...

One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm

Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P. aeruginosa infections, show high resistance to antibiotics; consequently, the biological disruption of mucoid P. aeruginosa biofilms is an attractive area of study for researchers. Algin...

The Role of Bacterial Vectors in the Expression of Human IFN-y Gene

Effects of biofilm formation in bacteria from different perspectives

Bacterial communities are able to form complex and three-dimensional biofilm structures. Biofilm formation is an ancient and integral component of the prokaryotic life cycle and a key factor for survival in diverse niches. In biofilms, bacterial lifestyle changes from free-floating cells to sessile cells. Presence in biofilms gives new traits to bacteria, which distinguish them from free cells....