Phytochemical Screening and In Vitro Bioactivities of the Extracts of Aerial Part of Evolvulus nummularius

Objective: To investigate in vitro bioactivities of methanol, ethyl acetate, chloroform, hexane, ethanol and aqueous extracts of E. nummularius and its phytochemical analysis. Methods: The antioxidant activity was determined by measuring the scavenging activity of DPPH radicals and H2O2. Cytotoxic activity was tested against hep G2 cell lines using MTT assay. Antimicrobial activity was assessed using the disc diffusion assays. The haemolytic activity was determined using agar diffusion techniques on blood agar plate, thrombolytic activity by clot disruption. Standard phytochemical screening tests for saponins, tannins, terepenoids, flavonoids, alkaloids and glycosides were also conducted. Results: Among the different extracts tested, ethyl acetate extract of E. nummularius showed potential in vitro bioactivities and phytochemical constituents. Conclusions: Further work will be carried out to find biologically active compounds that may serve as leads in the development of new pharmaceuticals.