Investigation of the inhibitory effects of HA-1077 and Y-32885 on the translocation of PKCbetaI, PKCbetaII and PKCzeta in human neutrophils.

نویسندگان

  • X Siomboing
  • B Gressier
  • T Dine
  • C Brunet
  • M Luyckx
  • M Cazin
  • J C Cazin
چکیده

To transmit the information inside the cell, one possibility is the action of an enzyme called kinase that phosphorylates other proteins. To study these enzymes, chemical compound synthesis was needed to know the function and the mechanism of activation. The major difficulty is creating a specific molecule for one kinase. In this study, we test the action of Rho-kinase inhibitors (HA-1077 and Y-32885) on protein kinase C (PKC) in the respiratory burst in the human polymorphonuclear neutrophils. We have shown that these compounds could inhibit the anion superoxide production. To prove their action on PKC, we have shown a decrease of binding of a specific ligand (phorbol-12,13-dibutyrate) with each inhibitor. During its activation, PKC was translocated from the cytoplasm to the plasmic membrane. We have also shown an inhibition of this translocation, proving an inhibition of PKC by HA-1077 and Y-32885.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation Uptake and Translocation of Iron Oxide Nanoparticles and Its Effect on Photosynthetic Pigmentation of Chrysanthemum (Chrysanthemum morifolium) ‘Salvador’

Recently, the use of superparamagnetic iron oxide nanoparticles (SPIONS) as a new and promising source of iron in agriculture has been suggested that further investigation is needed before extensive field use. In a greenhouse experiment, the effect of coated magnetite nanoparticles with humic acid (Fe3O4/HA NPs) was investigated on iron deficiency chlorosis and photosynthesis efficiency compare...

متن کامل

Evaluation of Growth Inhibitory and Apoptosis Inducing Activity of Human Calprotectin on the Human Gastric Cell Line (AGS)

Background: Calprotectin is cytotoxic agent that its anti-tumor effects are governed through suppression of topoisomerase II a key enzyme in apoptosis. In previous studies, cytotoxicity and apoptotic effects of calprotectin are shown on different cancer cell lines, but not human gastric cancer cell lines. In the present study, cytotoxicity and apoptotic effects of calprotectin on human gastric ...

متن کامل

Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCbetaII mRNA in vascular smooth muscle cells.

Acute hyperglycemia may contribute to the progression of atherosclerosis by regulating protein kinase C (PKC) isozymes and by accelerating vascular smooth muscle cell (VSMC) proliferation. We investigated acute glucose regulation of PKCbeta gene expression in A10 cells, a rat aortic smooth muscle cell line. Western blot analysis showed that PKCbetaII protein levels decreased with high glucose (...

متن کامل

Molecular Dissection Using Array Comparative Genomic Hybridization and Clinical Evaluation of An Infertile Male Carrier of An Unbalanced Y;21 Translocation: A Case Report and Review of The Literature

Chromosomal defects are relatively frequent in infertile men however, translocations between the Y chromosome and autosomes are rare and less than 40 cases of Y-autosome translocation have been reported. In particular, only three individuals has been described with a Y;21 translocation, up to now. We report on an additional case of an infertile man in whom a Y;21 translocation was associated wi...

متن کامل

Investigation of Eucalyptus and nanosilver as a new nanomixture for growth inhibition of E.coli

Nanotechnology is a new and specific method for therapy of diseases. Nanosilver particle is one of the functional fields of nanotechnology. It has been shown that these nanoparticles have antibacterial and antifungal properties. Different studied have been shown that nanoparticles may have important role in medicine. For example, it has been shown to have inhibitory effects on the treatment of ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Mediators of Inflammation

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2001