Isolation and Purification of a Serologically Active Phospholipid from Beef Heart
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Exchange of various phospholipids and of cholesterol between liposomes in the presence of highly purified phospholipid exchange protein.
The soluble fraction from beef heart homogenate is known to stimulate phospholipid exchange between cell membrane fractions. A purification procedure, consisting of pH adjustment to 5.1, gel filtration on Sephadex G-75, and isoelectric focusing between pH 4 and 6, yielded two fractions with high phospholipid exchange activities per mg of protein. The fraction with the highest specific activity ...
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