Equine assisted reproduction and embryo technologies
نویسندگان
چکیده
Assisted reproductive techniques in the horse have been only recently become available compared to other domestic species, in particular ruminants. The scarce availability of abattoir ovaries and the lack of interest from horse breeders and breed associations, and the anatomical and physiological differences have been the main reasons for this delay. Progressively though, the technology of oocyte maturation in vitro has been established especially after the application of ICSI to obtain in vitro fertilization. The parallel improvement of oocyte maturation conditions and embryo culture media has increased the rates of embryo development from in vitro matured and in vitro cultured ICSI embryos from 5-10% in the early studies to up to 26% in the latest under experimental conditions with abattoir derived oocytes. In 2003, the birth of the first cloned foal established the technology of somatic cell nuclear transfer. The largest set of data on non-surgical embryo transfer of in vitro produced embryos, from ICSI of in vitro-matured Ovum Pick Up (OPU) oocytes, and from somatic cell nuclear transfer, has been obtained in our laboratory. In the clinical context, where OPU and ICSI are applied for the treatment of female and or male infertility, the yield of embryos has been lower compared to experimental conditions. In conclusion, the basic procedures have been established for the use of assisted reproduction and somatic cell nuclear transfer to a degree suitable for clinical applications and the results have been replicated in several laboratories around the world.
منابع مشابه
P-175: Relationship between Dietary Fat Intake and Its Major Food Sources and Assisted Reproduction Parameters
Background: To examine the relative contributions of dietary fat intake level and its food sources and assisted reproduction parameters. Materials and Methods: A prospective study was conducted on 240 infertile women. In assisted reproduction treatment cycle, body mass index (BMI), physical activity (PA), calorie intake, fat consumption and major food sources were assessed. The number of retrie...
متن کاملApplication of Assisted Reproductive Technologies (ART) to Clinical Practice
In the past decade, assisted reproductive technologies (ART) such as oocyte transfer, intracytoplasmic sperm injection (ICSI), and nuclear transfer (cloning) have become effective enough for clinical use. ART such as oocyte transfer and ICSI allow production of foals from mares that cannot provide an embryo by standard embryo-transfer procedures. For the equine practitioner, application of ART ...
متن کاملNew assisted reproductive technologies applied to the horse industry: successes and limitations
The development of assisted reproduction technologies (ART) in the horse has been slow compared with that in other large domestic animals. Besides artificial insemination and embryo transfer, other technologies based on in vivo and in vitro procedures of embryo production (IVP) have appeared, but the success rates of equine IVP are still far from allowing their use in routine protocols. Intracy...
متن کاملHealth-Related Quality of Life and Primi-Gravid: A Comparative Study of Natural Conception and Conception by Assisted Reproduction Technologies (ARTs)
متن کامل
O-30: Impact of Environment Tobacco Smoke Exposure on Oxidative Stress in Antral Follicle and Assisted Reproduction Outcomes
Background: Cigarette smoke contains many oxidants and may alter the human reproduction by inducing oxidative stress (OS) in both active and passive smokers. This study was designed to evaluation the effect of environment tobacco smoke (ETS) exposure on oxidative stress in follicular follicle and assisted reproduction outcomes. Materials and Methods: A prospective study was carried out on 236 i...
متن کاملToward culture of single gametes: the development of microfluidic platforms for assisted reproduction.
During the last few decades in vitro production of mammalian embryos and assisted reproductive technologies such as embryo transfer, cryopreservation, and cloning have been used to produce and propagate genetically superior livestock. However, efficiencies of these technologies remain low. For these technologies to become more commercially viable, the efficiencies must improve. Despite this imp...
متن کامل