Linear Phase-gradient Imaging with Asymmetric Illumination based Differential Phase Contrast (AIDPC)
نویسندگان
چکیده
Differential Phase Contrast (AIDPC) Shalin B. Mehta,1,2,3,∗ and Colin J. R. Sheppard 1 Optical Bioimaging Lab, Division of Bioengineering, National University of Singapore, Block-E3A, #7-10, 7 Engineering Drive 1, Singapore 117574 2 NUS Graduate School for Integrative Sciences & Engineering (NGS), National University of Singapore 3 Liver Cancer Functional Genomics Lab, Humphrey Oei Institute of Cancer Research, National Cancer Center Singapore 4 Department of Biological Sciences, National University of Singapore ∗Corresponding author: [email protected], [email protected] This paper demonstrates how one could engineer the transfer function of AIDPC, a novel optical thickness (phase) gradient measurement method suitable for live cell imaging, for linear imaging of the specimen phase-gradient. OCIS codes: (110.4980) Partial coherence in imaging, (350.5030) Phase c © 2009 Optical Society of America
منابع مشابه
3D differential phase-contrast microscopy with computational illumination using an LED array.
We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational illumination with a programmable LED array. By capturing intensity images with various illumination angles generated by sequentially patterning an LED array source, we digitally refocus images through various depths via light field processing. The intensity differences from images taken at complementary illum...
متن کامل2016 - 10 - 01 3 D differential phase contrast microscopy
We demonstrate 3D phase and absorption recovery from partially coherent intensity images captured with a programmable LED array source. Images are captured through-focus with four different illumination patterns. Using first Born and weak object approximations (WOA), a linear 3D differential phase contrast (DPC) model is derived. The partially coherent transfer functions relate the sample’s com...
متن کاملQuantitative phase imaging and complex field reconstruction by pupil modulation differential phase contrast.
Differential phase contrast (DPC) is a non-interferometric quantitative phase imaging method achieved by using an asymmetric imaging procedure. We report a pupil modulation differential phase contrast (PMDPC) imaging method by filtering a sample's Fourier domain with half-circle pupils. A phase gradient image is captured with each half-circle pupil, and a quantitative high resolution phase imag...
متن کاملTransport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging.
Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving dir...
متن کاملALMA Memo 402 Illumination Taper Misalignment and Its Calibration
The alignment of the tapered illumination with the antenna's primary dish will not be perfect, and for ALMA has been speci ed as having an rms error of 0.1 dish radii at all frequencies, and the resulting image errors are therefore independent of frequency. If uncorrected, simulations indicate the e ects of this illumination o set will dominate both pointing and surface errors for ALMA's wide e...
متن کامل