Immune Response to Koi Herpesvirus (KHV) of Koi and Koi × Red Common Carp (Cyprinus carpio)
نویسندگان
چکیده
Koi herpesvirus (KHV), also known as Cyprinid herpes virus 3 (Cyprinid 3) is lethal disease in common carp and koi (Cyprinus carpio). Two different groups (KK and RK) were infected KHV by intraperitoneal injection. Fish for gene expression analysis were sampled at 0 h, 12 h, 24 h, 48 h and 72 h post infection (p.i). The results showed that two immune related gene, Interferons (INFs) ɑβ and Interleukin (IL)-12 p35 induced a high response in RK. The IL-12 p35 cytokine and Toll-like receptor (TLR) 9 were significantly high expressed on 48 h post infection (p.i) in RK as compared to the KK. The histopatological examination reveals focal necrosis in liver and infiltrate of lymphocytes in spleen of KK as compared to the RK. In immunohistochemistry analysis, the KHV protein high expressed in the infected kidney cell and slenocyte of KK. Therefore, the expression of IL-12 p35, IFN ɑβ and TLR 9 may provide a potentially genes related with KHV resistance in Koi and red common carp × koi.
منابع مشابه
Analysis of koi herpesvirus latency in wild common carp and ornamental koi in Oregon, USA.
Koi herpesvirus (KHV) infection is associated with high mortalities in both common carp (Cyprinus carpio carpio) and koi carp (Cyprinus carpio koi) worldwide. Although acute infection has been reported in both domestic and wild common carp, the status of KHV latent infection is largely unknown in wild common carp. To investigate whether KHV latency is present in wild common carp, the distributi...
متن کاملThe Current Problem : Koi Herpesvirus (khv)
Koi herpesvirus (KHV) is a pathogen agent that predominantly affects common and koi carp (Cyprinus carpio and Cyprinus carpio koi, respectively) causing disease and mass mortality in farmed and wild populations of these breeds. Following the first reports of KHV in Israel and Germany in 1998, the geographical range of this disease has become extensive. The disease has been spread to many countr...
متن کاملAn unusual koi herpesvirus associated with a mortality event of common carp Cyprinus carpio in New York State, USA.
Koi herpesvirus (KHV), a highly contagious and lethal virus that affects both koi (Cyprinus carpio koi) and common carp (Cyprinus carpio), was isolated in 1998 from two outbreaks of koi suffering mass mortality in New York State, USA, and in Israel. The disease had been described as early as 1996 in Europe. In July 2004, this virus was found associated with a mass mortality event in wild common...
متن کاملDetection of Koi Herpesvirus in Koi Cyprinus carpio in Taiwan
Mass mortality occurred in 2-year-old koi (fancy carp)Cyprinus carpio in Taiwan in 2002. Affected fish did not show any external signs except swollen gills. The consistent histological changes were observed in the gills; hyperplasia of epithelial cells, infiltration of eosinophilic granular cells and fusion of the secondary lamellae. Nega tively stained nucleocapsids were icosahedral and 112±1 ...
متن کاملCurrent knowledge on koi herpesvirus (KHV): a review
The first outbreaks of a disease connected with high mortality of common carp and koi carp caused by koi herpesvirus (KHV) were reported in 1998 in Israel and in the United States. Since then, several cases have been confirmed all over the world. At present, this viral disease is considered to be one of the most risky factors affecting populations of common carp and koi carp. Affected fish beco...
متن کامل