Development of bacteriophage in x-ray inactivated bacteria.
نویسندگان
چکیده
In previous studies (Labaw, Mosley, and Wyckoff, 1950a, b) from this laboratory we have shown that large yields of bacteriophage can be obtained from cultures of Escherichia coli which have been exposed to enough ultraviolet radiation to prevent their subsequent growth to form observable colonies when spread on fresh nutrient agar. In this work it was found that though the phosphorus metabolism of these bacteria is greatly depressed by the irradiation, it does not cease for two hours following exposure to the ultraviolet light; and it is during this time that bacteriophage can develop. It was observed also that during this interval of diminishing phosphorus uptake, the bacteria continue to grow and may even undergo one or two divisions. Infection with bacteriophage brings this growth to a prompt end though it may accelerate for a time the rate at which phosphorus is used by the infected bacterial system. The present paper records somewhat imilar observations made on systems consisting of X-rayed E. coli infected with bacteriophage. They are of interest in showing that bacteriophage grows even better on Xrayed than on ultraviolet irradiated bacteria and in bringing out certain other differences between bacteria "killed" by the two types of radiation.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 65 3 شماره
صفحات -
تاریخ انتشار 1953