Zinc Determined in 1O-/2L Serum or Urine Samples by Flameless Atomic Absorption Spectrometry
نویسندگان
چکیده
We describea preciseflameless atomicabsorption spectrometric method requiring only 10 tL ofsample, which thus permits repeated measurements in the neonate. Standard curves covering the range of 0 to 30 mg of zinc per liter, with standards in various matrixes (albumin, glycerol, serum, or nitric acid) hadslopesranging from 0.49 to1.17, relative tothatforaqueousstandards. We prepared low-zinc matrixes, which had slopes similar to that of serum, by dialyzing serum or a 50 gIL solution of albumin vs a buffered zinc-free dialysis fluid containing appropriate inorganic constituents. Use of thoroughly de-ionized water, reagent-grade chemicals, “ultrapure” nitric acid, specified disposable plastic ware, and appropriate pipet tip-rinsing techniques minimized extraneous contamination with zinc. Concentrations of zinc in serum calculated froma “rational-method” calibration algorithm fit tothestandard curveagreedwellwith independent determinations by flame atomic absorption spectrometry.
منابع مشابه
InterlaboratoryComparisons of Nickel Analyses in Urine by Atomic AbsorptionSpectrometry1’2’3’4
Two interlaboratory surveys of urine nickel analyses were performed in which eight or nine urine samples were distributed to laboratories in seven nations for measurements of nickel concentrations by atomic absorption spectrometry. In both surveys, wide discrepancies were observed in the values for urine nickel concentrations that were obtained in different laboratories by various analytical me...
متن کاملFeasibility evaluation of trace amount of zinc in urine samples using atomic absorption solidified floating organic drop micro-extraction technique
Background: The present study was carried out with the aim of extracting trace amounts of zinc in urine samples with ultrasound-assisted emulsification solidified floating organic drop micro-extraction (USAE-SFODME) method by flame atomic absorption spectrometry (FAAS). The efficiency was investigated using the solvent extraction volume, extraction pH, time sonication and temperature extraction...
متن کاملDirect determination of lead and cadmium in blood and urine by flameless atomic absorption spectrophotometry.
We describe procedures for direct determination of lead and cadmium in blood and urine by flameless atomic absorption spectrophotometry. Before analysis, the samples are pre-ashed in microboats in an ordinary laboratory oven. In this way, many samples can be prepared and pre-ashed simultaneously. We find the procedures presented in this work to be rapid, accurate, and precise.
متن کاملApplication of Cloud Point Preconcentration and Flame Atomic Absorption Spectrometry for the Determination of Cadmium and Zinc Ions in Urine, Blood Serum and Water Samples
A simple, sensitive and selective cloud point extraction procedure is described for the preconcentration and atomic absorption spectrometric determination of Zn and Cd ions in water and biological samples, after complexation with 3,3’,3”,3’”-tetraindolyl (terephthaloyl) dimethane (TTDM) in basic medium, using Triton X-114 as nonionic surfactant. Detection limits of 3.0 and 2.0 μg L and quantifi...
متن کاملAnalysis for platinum in biological material by flameless atomic absorption spectrometry.
A method for determining platinum in biological materials by flameless atomic absorption spectrometry has been developed for use in pharmacological studies of platinum antitumor compounds. Tissue digests are analyzed in a heated graphite atomizer, with use of background correction and ramp temperature charring. We have overcome problems of apparent loss of platinum in Pt-supplemented tissue sam...
متن کامل