23 Grafting of BET V 1 Epitopes onto its Homologue API G 1 Reveals Patient-Specific IgE Recognition Profiles
نویسندگان
چکیده
multi gene family), showed significant sequence and structural similarity to Cyn d 1. This protein was expressed in E. coli and the recombinant protein did not react with serum IgE from grass pollen allergic patients, suggesting that EXPB1 represented a non-allergenic homologue of grass group 1 allergens. It is proposed that differences in the amino acid sequence are responsible for the difference in the allergenicity profile of the Arabidopsis and grass pollen proteins. Conclusions: Our study provides valuable data for further investigations of the molecular basis of allergenicity and cross-reactivity of grass group 1 allergens.
منابع مشابه
Chimeras of Bet v 1 and Api g 1 reveal heterogeneous IgE responses in patients with birch pollen allergy
BACKGROUND Characterization of IgE-binding epitopes of allergens and determination of their patient-specific relevance is crucial for the diagnosis and treatment of allergy. OBJECTIVE We sought to assess the contribution of specific surface areas of the major birch pollen allergen Bet v 1.0101 to binding IgE of individual patients. METHODS Four distinct areas of Bet v 1 representing in tota...
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