Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
نویسندگان
چکیده
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
منابع مشابه
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ورودعنوان ژورنال:
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications
دوره 62 شماره
صفحات -
تاریخ انتشار 2006