Metabolism Studies with On-line HPLC and Mass Spectrometry (MS) Interfaced with the Flow Scintillation Analyzer (FSA)
نویسندگان
چکیده
In the biological sciences, mass spectrometry is one of the most popular methods employed for the determination of the molecular weights and structures of metabolites. The popularity of mass spectrometry is due to the high sensitivity of the analytical method and the possibility of obtaining molecular weights and structure of metabolites directly from high performance liquid chromatography (HPLC) effluents without further sample treatment. The traditional and more time-consuming methods of structure analysis, including chromatography of metabolites, followed by chromatogram fraction collection and purification prior to submission of isolated metabolites to mass spectrometry, have been applied for many years. Advanced techniques include the on-line mass spectral structural analysis of metabolites directly off the HPLC column after detection of peaks of interest. This application note describes the state-of-theart of on-line mass spectrometry of radioisotope-labeled metabolites following HPLC separation and FSA (Flow Scintillation Analysis) detection of chromatogram peaks of interest, also referred to as the hyphenated HPLCFSA-MS analysis. This paper will provide examples from the recent scientific literature that demonstrate the advantages of on-line radioactivity and mass spectral molecular structure analysis of radioisotope-labeled metabolites purified by HPLC obviating the time-consuming step of compound collection from HPLC fractions.
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