Culture of horse oocytes in vitro.

The equine oocyte: factors affecting meiotic and developmental competence.

There is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocyt...

In Vitro Maturation, Fertilization and Embryo Culture of Oocytes Obtained from Vitrified Auto-Transplanted Mouse Ovary

P-130: Efficacy of Commercial In Vitro Maturation Medium Is Similar to Home-made Medium for In Vitro Maturation of Human Oocytes

Background: In vitro maturation (IVM) of immature oocyte is a promising treatment option for infertile women.IVM procedures attempt to mimic the natural conditions of the follicular environment. The technique of IVM of oocytes has been successful after the first trial using culture of human oocytes in follicular fluid. The crucial part of this technique is the maturation process itself, and the...

P-90: The Effect of Nitric Oxide on Mouse Oocyte in Vitro Maturation in Two and Three Dimensional Conditions

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

O-9: Spindle and Chromosome Configurations of Human Oocytes Matured In vitro in Two Different Culture Media

Background: In vitro maturation can have deleterious effects on spindle formation and proper chromosome alignment in human oocytes and can be profoundly affected by culture conditions. This study compared the spindle presence and location with the maturation rate Abstracts of the 12th Royan International Congress on Reproductive Biomedicine 3 1 International Journal of Fertility & Sterility (IJ...

O-31: Follicular Fluid The Best Medium for In Vitro Maturation and In Vitro Fertilization

Background: Induction of in vitro maturation and development of immature oocyte is one of the methods that has been frequently used recently in artificial reproduction techniques and has drawn attention of many investigators. This method is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment ...