Correlation of Thyroid Hormone Dose-dependent Regulation of K-ras Proto oncogene Expression with Oncogene Activation by 3-Methylcholanthrene: Loss of Thyroidal Regulation in the Transformed Mouse Cell1

Previously, it has been demonstrated that thyroid hormone is an important cofactor of the initiation of oncogenesis in viro and in vitro. In order to determine the mechanism of thyroid hormone modulation of the initiation of carcinogenesis we have addressed the hypothesis that thyroid hormone regulates the expression of the critical protooncogene at the time of exposure to the carcinogen, and that the transcriptional activity of the protooncogene correlates with the ability of a carcinogen to "activate" the oncogene and thus modulate the subsequent transformation event. It has previously been shown that 3-methylchoIanthrene transfor mation of C3H/10T1/2 mouse embryo cells in culture is the result of activation of the k-ras oncogene. We report here that thyroid hormone modulates 3-methylcholanthrene transformation of C3H/10T1/2 cells in a dose-dependent manner that is similar to a thyroid hormone dosedependent modulation of k-rai-specific RNA levels in these cells. Further, nuclear transcriptional run-on experiments suggest that the thyroidalinduced changes in K-ras RNA levels are a result of a regulation of Kras transcription. These data support the hypothesis that thyroid hormone modulation of transformation is through regulation of protooncogene expression. It was of further interest to find that 3-methylcholanthrenetransformed ( 311/1(111/2 cells have lost the sensitivity to thyroid hor mone regulation of "activated" K-ras oncogene transcription and subse quent K-ras-specific RNA levels.