Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein
نویسندگان
چکیده
The objective of this study was to assess the association of polymorphisms in κ-cn, β-lg, β-lg 5′ flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in Chinese Holstein. Traits analyzed were 305 day standard milk yield, protein percentage, fat percentage, the ratio of fat percentage and protein percentage, pre-somatic cell count, somatic cell count, and somatic cell score, respectively. CSN1S2 locus was uninformative because only one genotype BB was found in Chinese Holstein. Allele frequencies of A and B in IGFBP-3 gene were 0.5738 and 0.4262 in Chinese Holstein population, which was different from reported Qinchuan cattle population. The genotypes of animals at IGFBP-3 locus significantly affected 305 day standard milk yield, protein percentage, and somatic cell score. The β-lg genotypes had a significant effect on protein percentage and the ratio of fat percentage and protein percentage. Polymorphism in β-lg 5′ flanking region was associated with 305 day standard milk yield, protein percentage, fat percentage, pre-somatic cell count, and somatic cell count. No significant associations of the polymorphism in κ-cn gene were observed for any trait. (
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