Biophysical study of Brucella abortus on electrochemical platform 1 Ajay

Study of biophysical interactions have been carried out using specific combination of proteins such as Brucella abortus cell envelop protein (as antigen) and its complementary antibody (raised in mice and rabbit). A best optimized composition of biocompatible CeO2 nano-octahedra and other biocompatible materials (chitosan) was used in this study. Linear range was found in the range of 6.25 μg/mL–100 μg/mL. Detection limit was found 0.1 ng/mL. By conventional plate ELISA it was possible to detect 50 μg/mL only. Experiments were conducted using 110 suspected patient serum samples and results were compared with ELISA and Rose Bengal Plate test (RBPT). We also studied the shelf life of prepared immunosensor. The shelf life of immunosensor was found to be 51 days. Index Term: Biophysics; Electrochemistry, ELISA; CeO2 nano-octahedra; CE-protein antigen Abbreviation: Ch, chitosan; GCE, Glassy carbon electrode; CE-protein antigen, Cell envelope protein antigen; ELISA, enzyme linked immunosorbent assay; RBPT, Rose Bengal Plat Test, PCR, Polymerase chain reaction; CAb, capturing antibody; RAb, revealing antibody; ALP, alkaline phosphatase; PBS, phosphate buffer saline; DEA, Diethanolamine; LPS, lipopolysaccharide; CV, Cyclic Voltammetry. PNPP, p-nitro phenyl phosphate, O.D., optical Density.