Quality Control for SureSelect Strand - Specifi c RNA Library Preparation Using the Agilent 2200
نویسنده
چکیده
This Application Note details a reliable and reproducible method for performing the recommended quality control (QC) steps of total RNA starting materials and corresponding cDNA libraries synthesized for RNA Sequencing (RNA-Seq) applications using the Agilent 2200 TapeStation system. Starting total RNA and fi nal cDNA libraries generated with the Agilent Strand Specifi c RNA library preparation workfl ow were analyzed for sample integrity and quantity on the 2200 TapeStation system, using the RNA ScreenTape and D1000 ScreenTape assays. Results were then compared to the Agilent 2100 Bioanalyzer system, using the RNA 6000 Nano and DNA 1000 assays. Analysis of the samples tested indicate that the performance of the RNA and D1000 ScreenTape assays for the 2200 TapeStation system are equivalent to the existing RNA 6000 Nano and DNA 1000 assays run on the 2100 Bioanalyzer system. These fi ndings show that the 2200 TapeStation system, when partnered with the RNA or D1000 ScreenTape assays, serve as an easy-to-use application for sample QC within the RNA-Seq workfl ow, offering fl exible sample throughput. Quality Control for SureSelect Strand-Specifi c RNA Library Preparation Using the Agilent 2200 TapeStation System
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