Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi
نویسندگان
چکیده
Beta-glucosidase is among the suite of enzymes produced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squamosus, Trametes versicolor, Lentinula edodes, and Grifola frondosa. All the WRF studied showed β-glucosidase activities. Significant variations in protein and carbohydrate contents were also recorded. Beta-glucosidase activities after 30 min of incubation ranged from 6.4 μg (T. versicolor) to 225 μg (G. frondosa). The calculated kinetic constant (Km) ranged from 0.47 μM (A. auricular-1120) to 719 μM (L. edodes-7). The Vmax depending on the kinetic transformation model ranged from 0.21 μg·min (T. versicolor) to 9.70 μg·min (G. frondosa-28). Beta-glucosidase activities also exhibited pH optima between 3.5 and 5.0 while temperature optima were between 60 ̊C and 70 ̊C with some media exhibiting a secondary temperature peak at 90 ̊C attributable to the presence of thermostable isoenzyme. WRF if appropriately screened and purified can be harnessed to potentially improve the bioconversion of cellulose to glucose and also facilitate efficient plant biomass biodegradation and production of useful plant bio-products.
منابع مشابه
Silver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کاملSilver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کاملComparative kinetic analysis of two fungal β-glucosidases
BACKGROUND The enzymatic hydrolysis of cellulose is still considered as one of the main limiting steps of the biological production of biofuels from lignocellulosic biomass. It is a complex multistep process, and various kinetic models have been proposed. The cellulase enzymatic cocktail secreted by Trichoderma reesei has been intensively investigated. beta-glucosidases are one of a number of c...
متن کاملSeveral Genes Encoding Enzymes with the Same Activity Are Necessary for Aerobic Fungal Degradation of Cellulose in Nature
The cellulose-degrading fungal enzymes are glycoside hydrolases of the GH families and lytic polysaccharide monooxygenases. The entanglement of glycoside hydrolase families and functions makes it difficult to predict the enzymatic activity of glycoside hydrolases based on their sequence. In the present study we further developed the method Peptide Pattern Recognition to an automatic approach no...
متن کاملPhorbol ester degradation in Jatropha seedcake using white rot fungi
White rot fungi are well known for their ability to degrade a wide range of xenobiotics due to their enzymatic systems. Therefore, the present investigation was aimed at screening ten different white rot fungi for degradation of phorbol esters from Jatropha seedcake (JSC). The JSC was fermented with pure cultures of white rot fungi for 20 days under solid state condition. All the white rot fung...
متن کامل