Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal
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چکیده
The Rockefeller University Press $30.00 J. Cell Biol. Vol. 192 No. 2 335–348 www.jcb.org/cgi/doi/10.1083/jcb.201009022 JCB 335 Correspondence to Laura A. Nilson: [email protected] Caroline Laplante’s present address is Dept. of Molecular, Cell, and Developmental Biology, Yale University, New Haven, CT 06520. Abbreviations used in this paper: ANC, actin-nucleating center; AP, anterior– posterior; Baz, Bazooka/PAR-3; Dia, Diaphanous; DME, dorsal-most epidermal; DV, dorsal–ventral; Ed, Echinoid; Ena, Enabled/VASP; F-actin, filamentous actin; Fmi, Flamingo; MARCM, mosaic analysis with a repressible cell marker; MHC, myosin heavy chain; pAS, peripheral amnioserosa; pMLC, phosphorylated myosin II light chain; UAS, upstream activating sequence. Introduction
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Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure
During Drosophila melanogaster dorsal closure, lateral sheets of embryonic epidermis assemble an actomyosin cable at their leading edge and migrate dorsally over the amnioserosa, converging at the dorsal midline. We show that disappearance of the homophilic cell adhesion molecule Echinoid (Ed) from the amnioserosa just before dorsal closure eliminates homophilic interactions with the adjacent d...
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