Assessment of the mosaic structure in the Helicobacter pylori cagA gene 3'-region using an improved polymerase chain reaction-based assay.
نویسندگان
چکیده
The mosaic structure of the cagA gene has been suggested to affect Helicobacter pylori CagA-associated pathogenesis. An improved polymerase chain reaction assay allowed for a rapid and detailed molecular analysis of the cagA gene 3'-region in a single amplification step, followed by amplicon sequencing using universal M13 and T7 sequencing primers.
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ورودعنوان ژورنال:
- Diagnostic microbiology and infectious disease
دوره 73 3 شماره
صفحات -
تاریخ انتشار 2012