Improved method of embedding with epoxy resin 'Quetol 651' for both light and electron microscopic observation of identical sites in semi-thin sections.

on a conventional ultramicrotome. Semi-thin sections on grids were stained with the Giemsa method and methylene blue-azure II-basic fuchsin. Staining without removal of the embedding matrix was sharp and brilliant. These stained sections were observed under a light microscope. For electron microscopy, they were doubly stained with uranyl acetate and lead salt. Identical sites on such sections could be distinctly examined with an accelerating potential of 100 kV at low magnification (250-1,500 times) using LEM-2000, a combined light and electron microscope. Thus, photomicrographs electron micrographs of identical sites on tissue samples could be compared exactly.