Pressure-Activated Microsyringe Composite Scaffold of Poly(L-lactic acid) and Carbon Nanotubes for Bone Tissue Engineering
نویسندگان
چکیده
Tissue engineering is an innovative interdisciplinary field in which bioengineers and life scientists try to regenerate and reproduce natural tissues through the use of biodegradable structures, called scaffolds, with the aim of mimicking the specific tissue extracellular matrix (ECM). Carbon nanotubes (CNTs) offer a natural platform for obtaining composite microfabricated scaffolds thanks to their excellent mechanical properties and their good biocompatibility. In this study, we microfabricated three-dimensional (3D) scaffolds by mixing poly(L-lactic acid) (PLLA) and multiwalled carbon nanotubes (MWCNTs) for bone tissue engineering. We measured their mechanical properties and studied their biocompatibility with human fetal osteoblasts (hFOB 1.19). The 3D microfabricated PLLA/MWCNTs nanocomposite scaffolds showed higher stiffness and cell viability than the pure 3D microfabricated PLLA scaffolds. The results of this preliminary work suggest that biopolymer/CNT microcomposites and nanocomposites could be used as effective building blocks to replace ECMs in bone tissue engineering applications. The final goal is the creation of innovative scaffolds for implants and tissue regeneration. VC 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 129: 528–536, 2013
منابع مشابه
Comparison of Proliferation and Osteoblast Differentiation of Marrow-Derived Mesenchymal Stem Cells on Nano- and Micro-Hydroxyapatite Contained Composite Scaffolds
Bones constructed by tissue engineering are being considered as valuable materials to be used for regeneration of large defects in natural bone. In an attempt to prepare a new bone construct, in this study, proliferation and bone differentiation of marrow-derived mesenchymal stem cells (MSCs) on our recently developed composite scaffolds of nano-, micro-hydroxyapatite/ poly(l-lactic acid) were ...
متن کاملCytocompatibility of a conductive nanofibrous carbon nanotube/poly (L-Lactic acid) composite scaffold intended for nerve tissue engineering
The purpose of this study was to fabricate a conductive aligned nanofibrous substrate and evaluate its suitability and cytocompatibility with neural cells for nerve tissue engineering purposes. In order to reach these goals, we first used electrospinning to fabricate single-walled carbon-nanotube (SWCNT) incorporated poly(L-lactic acid) (PLLA) nanofibrous scaffolds and then assessed its cytocom...
متن کاملPLLA/HA Nano composite scaffolds for stem cell proliferation and differentiation in tissue engineering
Abstract Due to their mulitpotency, Mesenchymal stem cells (MSCs), have the ability to proliferate and differentiate into multiple mesodermal tissues. The aim of this study was to isolate MSCs from human Umbilical Cord (hUCMSCs) to determine their osteogenic potential on nanofibrous scaffolds. To this end, Poly (L-lactic acid) (PLLA)/Nano hydroxyapatite (HA) composite nanofibrous scaffolds were...
متن کاملDifferentiation of Human Mesenchymal Stem Cell into Chonderocyte Like Cells 3D Poly Lactic Acid Glycosaminoglycan (PCL-GAG) Nano Fibre Scaffold
Introduction: Failure of human body tissue and organs is believed to be one of the most important health problems all over the world. The great challenge for tissue engineers is to optimize suitable systems to separate, proliferate and differentiate the cells so that they can set out to create tissue by a harmonic 3-D growth. Therefore, the tissue engineers must provide an environment like the ...
متن کاملMechanical performance of three-dimensional bio- nanocomposite scaffolds designed with digital light processing for biomedical applications
Introduction: The need for biocompatible and bioactive scaffolds to accelerate the regeneration and repair of fractured bones has been considered for bone tissue engineering applications during recent decades. The new methods were developed to produce scaffolds to improve the tissue quality, size of cavities, control the porosity and increase the scaffold compressive strength u...
متن کامل