Bacterial metabolism of thiamine. II. The isolation and characterization of 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methylthiazole-5-acetic acid (thiamine acetic acid) as an intermediate in the oxidation of thiamine.

The compound 3-(2’-methyl-4’-amino-S’-pyrimidylmethyl)-4-methylthiazole-S-acetic acid (thiamine acetic acid) has been identified as an intermediate in the metabolism of thiamine by a soil microorganism. Evidence is presented which indicates the compound 2-oxy-3-(2’-methyl-4’-amino5’-pyrimidylmethyl)-4-methylthiazole-S-acetic acid (2-oxythiamine acetic acid) is also an intermediate in the metabolism of thiamine by this microorganism.