A novel mutation for low erucic acid found in Brassica napus cv. Zhongshuang No.9: gene cloning and molecular characterization
نویسندگان
چکیده
The FAE1 genes isolated from Zhongshuang 9 both harbored a deletion of 4 nucleotides between T1366 and G1369, leading to a frameshift mutation and the premature stop of translation after 466 amino acid residues. This was evidenced and supported by heterologous expression of FAE1 genes in yeast and immunoblot analysis. A RT-PCR and Western blot experiments with the developing seeds of Zhongshuang 9 indicated that the frameshift FAE1 genes could be transcribed normally but failed to translate proteins to form a functional complex. It is considered that Zhongshuang 9 is the second source of LEA gene following the first finding of the LEA feed rape variety LIHO in 1960s.
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