Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S -transferase, an evolved toluene o -monooxygenase, and g -glutamylcysteine synthetase
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چکیده
Received 30 July, 2003; revised 26 November, 2003; accepted 19 December, 2003. *For correspondence. E-mail [email protected]; Tel. ( + 1) 860 486 2483; Fax ( + 1) 860 486 2959. Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S -transferase, an evolved toluene o -monooxygenase, and g -glutamylcysteine synthetase
منابع مشابه
Proposal to Present #12e: A Proteomic Approach to Identify Physiological Changes in E. coli Metabolically Engineered for Enhanced TCE Degradation and Toxicity Reduction
Trichloroethylene (TCE) and other chlorinated ethenes are environmental pollutants frequently found in groundwater. Anaerobic biodegradation of these chemicals can produce more toxic compounds, while aerobic degradation is limited to cometabolism and involves the production of an intermediate epoxide that is cytotoxic, eventually inactivating the bacterium. Toluene ortho-monoxygenase (TOM) was ...
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