Phage typing scheme for group D streptococci isolated from human urogenital tract.
نویسندگان
چکیده
Streptococci of Lancefield group D were isolated from 52.2% of pharyngeal, 55.5% of urethral, 56.6% of endocervical, and 75.4% of ano-rectal specimens. Seventeen phages isolated from sewage and urogenital specimens were selected for phage typing. Four of these lysed only the strains of Streptococcus faecalis and its variety liquefaciens. Another six phages lysed only the strains of Streptococcus faecium and its variety durans. With the help of seven bacteriophages, 346 of 384 (90.1%) strains of S. faecalis and its variety liquefaciens could be classified into 27 types. Similarly, with the help of 10 other bacteriophages, 97 of 117 (82.9%) strains of S. faecium and its variety durans could be grouped into 22 types. In 72 of 87 (82.7%) cases, similar phage types were obtained at different culture sites of the same individual.
منابع مشابه
Carriage of group B streptococci in rectum and urogenital tract of pregnant women.
One hundred pregnant women in third trimester were screened for the carriage of group B streptococci (GBS) in a prospective study. Rectal and urogenital samples were collected, 25% women were positive for non group B beta haemolytic streptococci. Twelve (48%) were positive at single site and 13 (52%) at more than one site. Beta haemolytic streptococci isolated were non-groupables in 19 (76%) an...
متن کاملMethod for phage typing group A type 49 streptococci.
A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type 49 streptococci isolated from 10 worldwide sources were examined by this meth...
متن کاملPatterns of carriage of group B streptococci in genitourinary medicine clinic patients.
Anogenital carriage of group B streptococci was found in 46% (57/125) of men and 38% (68/179) of women attending a genitourinary medicine clinic. Colonisation with group B streptococci was more common in patients who had a history of more than one sexual partner in the preceding three months, but was not related to any previous sexually transmitted infection. Group B streptococci were isolated ...
متن کاملUse of 5-Enolpyruvylshikmate-3-Phosphate Synthase Encoding Gene for Typing of Staphylococcus aureus Isolated from Skin and Urinary Tract Infections of Human
Objective(s) Staphylococcus aureus is both a successful human commensal and a major pathogen. In this study we investigated the genetic diversity of 26 S. aureus isolates recovered from human skin and urinary tract infections. Materials and Methods Typing procedure for the studied S. aureus isolates was performed based on PCR amplification of the aroA gene, which encodes the enzyme 5-enolpyr...
متن کاملA globally disseminated M1 subclone of group A streptococci differs from other subclones by 70 kilobases of prophage DNA and capacity for high-frequency intracellular invasion.
The M1inv+ subclone of M1 group A streptococci that spread globally in the late 1980s and early 1990s was previously identified by restriction fragment length polymorphism (RFLP), M protein, and SpeA exotoxin sequence analyses. Strains representing this subclone were characterized with regard to carriage of bacteriophage and capacity to invade cultured human epithelial cells. The M1inv+ subclon...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 2 4 شماره
صفحات -
تاریخ انتشار 1975